The 2012 Louis-Jeantet Prize for Medicine

1/25/2012

The 2012 Louis-Jeantet Prize for Medicine is awarded to the German specialist in systems biology Matthias Mann, Director of the Department of Proteomics and Signal Transduction at the Max-Planck-Institute of Biochemistry in Martinsried, and to the British biologist Fiona Powrie, Sidney Truelove Professor of Gastroenterology at the University of Oxford and Head of the Experimental Medicine Division of the Nuffield Department of Medicine at the John Radcliffe Hospital, University of Oxford. Continue reading

Posted in For the Professional, For the Student, Isotope Research, Naturally Occurring, People, Research, Stable Isotopes, Uses for Isotopes | Leave a comment

Technical note: Interpreting stable carbon isotopes in human tooth enamel: An examination of tissue spacings from South Africa

1/25/2012

Abstract

Stable isotope analysis of skeletal tissues is widely used in archeology and paleoanthropology to reconstruct diet. In material that is poorly preserved or very old, the tissue of choice is frequently tooth enamel, since this is less susceptible to diagenesis. The relationships between carbon isotope ratios in tooth enamel, bone collagen, and bone apatite are, however, not well understood. To elucidate these, we have measured all three indicators in archeological humans from the western and southern Cape coastal regions of South Africa. Am J Phys Anthropol, 2012. © 2012 Wiley Periodicals, Inc.

– by Emma Loftus and Judith Sealy

Click here for more information.

Posted in For the Professional, Isotope Research, Naturally Occurring, Research, Stable Isotopes, Technical Papers, Uses for Isotopes | Leave a comment

Radioactive medicine without the nuclear headache

1/24/2012

A made-in-Canada solution to our medical-isotope problem could come from a machine with a name that could have been pulled straight from the pages of a science fiction novel: the cyclotron.

“It was really pooh-poohed, this idea of using cyclotrons; they said there was no way we could produce enough in a commercially meaningful way,” says John Wilson, the cyclotron facilities manager at the University of Alberta’s Cross Cancer Institute. Continue reading

Posted in Cyclotron Produced, For the Student, Medical, Radioisotopes, Reactor Produced, Uses for Isotopes | Leave a comment

Isotope effects in high-Tc cuprate superconductors as support for the bipolaron theory of superconductivity

1/24/2012

Abstract

We provide a unified parameter-free explanation of the observed oxygen-isotope effects on the critical temperature, the magnetic-field penetration depth and on the normal-state pseudogap for underdoped cuprate superconductors within the framework of the multi-(bi)polaron theory with strong Coulomb and Fröhlich interactions. We also quantitatively explain the measured critical temperature and the magnitude of the magnetic-field penetration depth. This paper thus represents an important support for the bipolaron theory of high-temperature superconductivity, compatible with many other independent observations.

– by A. S. Alexandrov and G. M. Zhao

Click here for more information.

Posted in For the Professional, Naturally Occurring, Research, Stable Isotopes, Technical Papers, Uses for Isotopes | Leave a comment

Post-Doc Position

1/24/2012

The Department of Geological Sciences at Indiana University invites applications for a full-time postdoctoral research position aligned with a NASA-funded project entitled Shallow-Borehole Array for Measuring Greenland Emission of Trace Gases as an Analogue for Methane on Mars.

We seek an individual with demonstrated expertise in stable isotope geochemistry and geomicrobiology who is interested in isotope systematics associated with methane cycling in permafrost environments. The Stable Isotope Research Facility at Indiana University includes several gas-source mass spectrometers and a Nu Plasma II multi-collector ICP-MS, housed in a new 56,000 sq ft multidisciplinary research facility on the Bloomington campus.

Click here for more information.

Posted in Careers, For the Professional, Naturally Occurring, Research, Stable Isotopes, Uses for Isotopes | Leave a comment

Measurement of Hepatic Protein Fractional Synthetic Rate with Stable Isotope Labeling Technique in Thapsigargin Stressed HepG2 Cells

1/24/2012

Abstract

Severe burn-induced liver damage and dysfunction is associated with endoplasmic reticulum (ER) stress. ER stress has been shown to regulate global protein synthesis. In the current study, we induced ER stress in vitro and estimated the effect of ER stress on hepatic protein synthesis. The aim was two-fold: (1) to establish an in vitro model to isotopically measure hepatic protein synthesis and (2) to evaluate protein fractional synthetic rate (FSR) in response to ER stress. Human hepatocellular carcinoma cells (HepG2) were cultured in medium supplemented with stable isotopes 1,2-13C2-glycine and L-[ring-13C6]phenylalanine. ER stress was induced by exposing the cells to 100 nM of thapsigargin (TG). Cell content was collected from day 0 to 14. Alterations in cytosolic calcium were measured by calcium imaging and ER stress markers were confirmed by Western blotting. The precursor and product enrichments were detected by GC-MS analysis for FSR calculation. We found that the hepatic protein FSR were 0.97±0.02 and 0.99±0.05%/hr calculated from 1,2-13C2-glycine and L-[ring-13C6]phenylalanine, respectively. TG depleted ER calcium stores and induced ER stress by upregulating p-IRE-1 and Bip. FSR dramatically decreased to 0.68±0.03 and 0.60±0.06%/hr in the TG treatment group (p<0.05, vs. control). TG-induced ER stress inhibited hepatic protein synthesis. The stable isotope tracer incorporation technique is a useful method for studying the effects of ER stress on hepatic protein synthesis.

– by Juquan Song, Xiao-jun Zhang, Darren Boehning, Natasha C. Brooks, David N. Herndon and Marc G. Jeschke

Click here for more information.

 

 

Posted in For the Professional, Isotope Research, Naturally Occurring, Research, Stable Isotopes, Technical Papers, Uses for Isotopes | Leave a comment

Postdoctoral Scholar on sources and cycling of non-photosynthetic carbon in plants

1/24/2012

The Department of Earth System Science at the University of California, Irvine seeks applicants for a postdoctoral scholar position in experimental research on sources and cycling of non-photosynthetic carbon in plants. The successful candidate will work closely with Drs. Guaciara dos Santos and Kathleen Treseder to design and test hypotheses regarding the role of carbon within high plant biosilica structures known as phytoliths. The project involves working with carbon isotopes and nanotechnological particles coupled with image techniques, in addition to collaborations with the Stable Isotope laboratory team in CEREGE/France and the Soils and Sediments Analysis Laboratory, University of Wisconsin-Madison.

Click here for more information.

Posted in Careers, For the Professional, Isotope Research, Research, Stable Isotopes, Uses for Isotopes | Leave a comment

Activities of gamma-ray emitting isotopes in rainwater from Greater Sudbury, Canada following the Fukushima incident

1/24/2012

Abstract

We report the activity measured in rainwater samples collected in the Greater Sudbury area of eastern Canada on 3, 16, 20, and 26 April 2011. The samples were gamma-ray counted in a germanium detector and the isotopes 131I and 137Cs, produced by the fission of 235U, and 134Cs, produced by neutron capture on 133Cs, were observed at elevated levels compared to a reference sample of ice-water. These elevated activities are ascribed to the accident at the Fukushima Dai-ichi nuclear reactor complex in Japan that followed the 11 March earthquake and tsunami. The activity levels observed at no time presented health concerns.

– by B. T. Cleveland, F. A. Duncan, I. T. Lawson, N. J. T. Smith and E. Vazquez-Jauregui

Click here for more information.

Posted in For the Professional, Industrial, Isotope Research, Radioisotopes, Reactor Produced, Research, Technical Papers, Uses for Isotopes | Leave a comment

The chemistry of exploding stars

1/20/2012

Star dust from a supernova. The electron microscopic image shows a silicon carbide grain from the meteorite Murchinson. The approximately one micrometre small grains originate from a supernova as an isotopic analysis has shown. Isotopes are forms of an element with different weights. Picture: Peter Hoppe, Max Planck Institute for Chemistry © Peter Hoppe, MPI for Chemistry.

Fundamental chemical processes in predecessors of our solar system are now a bit better understood: An international team led by Peter Hoppe, researcher at the Max Planck Institute for Chemistry in Mainz, has now examined dust inclusions of the 4.6 billion years old Murchison meteorite, found in 1969, using a very sensitive method. The stardust grains originate from a supernova, and are older than our solar system. The scientists discovered chemical isotopes, which indicate that sulfur compounds such as silicon sulfide originate from the ejecta of exploding stars. Sulfur molecules are central to many processes and important for the emergence of life.

Models already predicted the formation of sulfur molecules in the ejecta of exploding stars – the supernovae. Scientists from Germany, Japan and the U.S. now provided evidence to substantiate the theory with the help of isotope analyses of stardust from meteorites.

The team around the Mainz Max Planck researcher Peter Hoppe initially isolated thousands of about 0.1 to 1 micrometre-sized silicon carbide stardust grains from the Murchison meteorite, which was already found on Earth in 1969. The stardust grains originate from a supernova, and are older than our solar system. The researchers then determined with a highly sensitive spectrometer, the so-called NanoSIMS, the isotopic distribution of the samples. With this technique an ion beam is shot onto the individual stardust grains and releases atoms from the surface. The spectrometer then separates them according to their mass and measures the isotopic abundances. Isotopes of a chemical element have the same number of protons but different numbers of neutrons.

In five silicon carbide samples the astrophysicists found an unusual isotopic distribution: They measured a high amount of heavy silicon and a low amount of heavy sulfur isotopes, a result that does not fit with current models of isotope abundances in stars. At the same time they were able to detect the decay products of radioactive titanium which can be produced only in the innermost zones of a supernova. This proves that the stardust grains indeed derive from a supernova.

A proof for the model of the chemistry of the ejecta of supernovae

“The stardust grains we found are extremely rare. They represent only about the 100 millionth part of the entire meteorite material. That we have found them is very much a coincidence – especially since we were actually looking for silicon carbide stardust with isotopically light silicon,” says Peter Hoppe. “The signature of isotopically heavy silicon and light sulfur can only be plausibly  explained if silicon sulfide molecules were formed in the innermost zones in the ejecta of a supernova.” Afterwards, the sulfide molecules were enclosed in the condensing silicon carbide crystals. These crystals then reached the solar nebula around 4.6 billion years ago and were subsequently incorporated into the forming planetary bodies. They finally reach the Earth in meteorites which are fragments of asteroids.

Carbon monoxide and silicon monoxide were already detected in infrared spectra of the ejecta of supernova explosions. Although models predicted the formation of sulfur molecules, it has not yet been possible to prove this. The measurements on silicon carbide stardust now provide support to the predictions that silicon sulfide molecules arise a few months after the explosion at extreme temperatures of several thousand degrees Celsius in the inner zones of supernova ejecta.

The meteorite studied was named after the Australian city of Murchison, where it was found in 1969. For astronomers, it is an inexhaustible diary about the formation of our solar system, as it has remained almost unaltered since its formation. Besides the stardust inclusions from the ejecta of a supernova Murchison also transported dust to the Earth which has been formed in the winds of giant red stars. Through further analyses, the researchers hope to learn more about the origin of their parent stars.

- Provided by Max-Planck-Gesellschaft

Click here for more information.

Posted in For the Professional, For the Student, Isotope Research, Naturally Occurring, Research, Stable Isotopes, Uses for Isotopes | Leave a comment

Hair database will help forensic investigators identify bodies

1/20/2012

Forensic tool kits will soon be expanded by the slimmest of threads: human hair.

Sound like an episode of CSI?

It is the kind of science American TV crime solvers have been using for years to identify John and Jane Does. And now two Canadian scientists say the reality is right around the corner.

University of Ottawa researchers Michelle Chartrand and Gilles St-Jean are building a national database of hair that will be used a forensic tool to help investigators identify human remains.

University of Ottawa researchers Gilles St-Jean and Michelle Chartrand have spent years collecting and analyzing hair from across the country to build a database that will help forensic investigators identify unidentified remains. They hope to have the database up and running by the end of 2012.

On television crime shows, there is already a database for everything, Chartrand says. “In reality, those databases don’t exist. That’s what we’re trying to build here.”

And the clues to building this database are in the water.

When water is consumed, it leaves a chemical fingerprint in hair. And because people tend to drink and cook with their local water, which can vary by region, the signature left on the hair will be geographically unique.

“This is a new tool to help investigators who’ve hit a wall. Sometimes they have no idea where to look,” says St-Jean. “You can get DNA from a body that you’ve found, but if that person never wound up in a DNA database, it’s a useless piece of information.”

Researchers can tell where a person has been by studying the hydrogen and oxygen in the hair. Specifically, they analyze stable isotopes — different forms of the same chemical element — in the hair.

Because hair retains isotopic information, and grows about one centimetre each month, it can provide a personal chronology of where a person has been. If a person moves across the country over the course of a year, that movement will be reflected in the last 12cms of hair growth.

The longer the hair, the longer the trail of footsteps. It’s like having a passport that’s been stamped along the way.

So if an unidentified body is found in downtown Toronto, the person’s hair may indicate they’re not from the city, but a resident of a remote community in northern Ontario — a detail that could prove useful to investigators.

“What stable isotope analysis can do is help us focus our investigation,” says Superintendent John House of the Royal Newfoundland Constabulary. “With a lot of these cases, we have no idea who they are. We don’t know if they’re transients who’ve come in or even if they’re foreigners.”

As of Dec. 1, 2011, there were 205 unsolved unidentified bodies in the OPP database and about 600 unidentified bodies in Canada.

A national database, “would be a very important tool for police,” says House.

The science behind St-Jean and Chartrand’s research has already been applied in Canadian cases. Back in 2006, House suggested stable isotope analysis be used for the first time in a Newfoundland cold case: the Minerals Road skull.

In 2001, hikers trekking through the woods of Conception Bay South, NL, stumbled upon a human skull, wrapped in a plastic bag. Investigators were stumped, despite exhausting many investigative procedures.

After learning about researchers in Europe doing stable isotopic analysis, House sent them samples of the skull and hair, which was 17cms long.

Scientists determined the male victim had lived for extended periods in southern Ontario or southern Quebec, and/or Atlantic Canada. Or, the north-eastern United States. They also noted a blip in the isotopic signature, suggesting he had visited Newfoundland for a brief period about 13 months prior to his death.

Other testing helped estimate the man’s age — he had been born between 1955 and 1961 — and decapitated between 1995 and 1997.

The tests generated new leads, but not enough to crack the case. The Minerals Road skull remains unidentified.

Although the Ottawa scientists are still finalizing their research, they’ve already worked a handful of cold cases with the RCMP and provincial police forces in Ontario and Quebec.

Among the cases is that of so-called Madame Victoria.

In 2001, a badly-decomposed body of a woman in her 50s was found in a wooded area near Montreal’s Royal Victoria Hospital. It’s believed her remains were there for two years.

In January 2010, the coroner’s office sent Chartrand samples of the woman’s hair — It was 43 cms-long, providing 43 months of information. With the hair, Chartrand discovered the woman had moved to seven different locations in the last 43 months of her life, travelling from northern Ontario or Quebec and moving south to Montreal. The longest time she had spent in one place was seven months.

The hair also revealed that in the last five months of her life, she may have been extremely ill, and had likely lost a great deal of weight — a telling detail that helped the facial reconstruction artist.

While the hair analysis didn’t crack the case, Chartrand provided investigators with some insight into Madame Victoria’s movements, diet and health.

To build a database, showing the isotopic components in hair found from coast to coast, Chartrand spent four years travelling across Canada. She collected about 600 different locks of hair, along with samples of the local tap water. In order to obtain a stable signal specific to the region, she sought participants who rarely, if ever, travelled.

This way, when an unidentified body is found the individual’s hair can be compared against the database to try and determine where he or she may have come from.

“We’re always interested in anything that can provide additional information to help investigators,” says RCMP forensic scientist Ron Fourney, who says that even though isotopic hair analysis is still in the early stages of research, “It’s very exciting.”

“It’s another tool in the forensic tool box, a very important one and one that we haven’t seen before,” says Fourney, director of National Services and Research, which falls under the Forensic Science and Identification Services of the RCMP. The agency is working with the scientists to build the database, a project that is being funded by the government agency Centre for Security Science.

In Canada, the hydrogen isotope signals in water vary according to latitude and altitude. As you move north, they tend to become less heavy — a signal that will be reflected in the hair of those who live there.

While this method of hair analysis is gaining popularity in forensic science cases where DNA testing and other traditional means of investigation have shed little light, it does have its own geographical limits.

Scientists can identify regions, but not cities. For instance, they can tell whether someone came from southern or northern Ontario, but can’t pinpoint Kitchener or Kapuskasing.

Also, areas in a region that get drinking water from the same source are lumped together. For instance, the hydrogen isotope signals in hair of people living in Hamilton, Toronto and Kingston will be similar because those cities all rely on Lake Ontario for water.

“When I moved from Toronto to Ottawa and started drinking the local water, my signal started to change immediately,” says Chartrand, explaining that Ottawa gets its drinking water from the Ottawa River, the source of which comes from the north.

Chartrand and St-Jean are still determining the impact hair dye has on isotope values. But, so far, research indicates there’s no change for hair that has been bleached or dyed blond. They’ll also need to analyze, what difference, if any, is there if someone drinks bottled water, or has a daily glass of say Australian wine.

Isotopic signals from other chemicals — carbon and nitrogen — reveal information about a person’s diet and health.

“Everything we consume goes into making our body tissue, including our hair,” says Chartrand. “It is very true that you are what you eat.”

– by Isabel Teotonio, TheStar.com

Click here for more information.

Posted in For the Student, Isotope Research, Naturally Occurring, Research, Stable Isotopes, Uses for Isotopes | Leave a comment